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Input File Requirements

#Locus name	Allele number	Allele Frequency	Observed?
vWA	11	0.2	1
vWA	12	0.2	x
vWA	13	0.6
FGA	13	0.3	xx
FGA	14	0.2	o
FGA	15	0.5

The input file has to be a comma-separated values text file (csv file). So the information in the table above should be formatted like:

#Locus name,Allele number,Allele Frequency,Observed?
vWA,11,0.2,1
vWA,12,0.2,x
vWA,13,0.6,
FGA,13,0.3,1
FGA,14,0.2,x
FGA,15,0.5,


• The header row is not necessary. If one is used in the file, make sure that it starts with a "#"
• All observed AND non-nobserved alleles and their corresponding fequencies need to be in the file
• The locus name can be anything
• The allele number can be anything
• The allele frequency has to be a number between 0 an 1
• Any character can be used to mark the allele as observed

Download example file

Input Limitations

The RMNE calculation allowing for allelic drop-out is a combinatorial mathematics problem resulting in 2n!/(2n-r)!r! combinations with n the number of loci and r the number of DO. Because of this, the calculation time is dependent both on n and r. The website currently accepts between 1 and 200 loci. The maximum number of allowed DO that can be calculated on the website is restricted to calculations that do not exceed 5 minutes calculation time. The calculation time is estimated beforehand using the formula 2n!/(2n-r)!r! with n the number of loci and r the number of DO. When the calculation time is estimated to be too high, the website returns the error message "This calculation would take too much time. Please reduce the number of analysed loci or the number of allowed DO."

Refresh

When starting the calculation, the webpage will automatically show the results. For small calculations e.g. the example input file with 2 loci, the calculation will finish faster that the auto-refresh rate. You can save time by hitting the refresh button of your browser.

Error messages

This calculation would take too much time. Please reduce the number of analysed loci or the number of allowed DO.

The allowed number of dropout should be less than twice the number of loci.

All alleles should have a name and a frequency, otherwise the error message "This field is required." is displayed.

Developed by LabFBT, Ghent University, Belgium.